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真核翻譯起始因子4A1(EIF4A1)重組蛋白 真核生物的翻譯啟動(dòng)需要組裝80S核糖體復(fù)合體。真核起始因子(eIFs)用于一系列反應(yīng),導(dǎo)致80S核糖體組裝和翻譯啟動(dòng)。哺乳動(dòng)物真核翻譯起始因子4F(eIF4F)是一種蛋白質(zhì)復(fù)合物,含有eIF4A、eIF4E和eIF4G,在5'帽基序處結(jié)合mRNA,并將43S核糖體預(yù)起始復(fù)合物招募到合格的轉(zhuǎn)錄物中。
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品牌 | 信裕生物 | CAS | 詳見(jiàn)說(shuō)明書(shū) |
---|---|---|---|
分子式 | 詳見(jiàn)說(shuō)明書(shū) | 純度 | > 95% |
分子量 | 34.7kDa | 貨號(hào) | XY91787RPHu |
規(guī)格 | 50µg/100µg | 供貨周期 | 一周 |
主要用途 | SDS-PAGE; WB | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
Recombinant Eukaryotic Translation Initiation Factor 4A1 (EIF4A1)
真核翻譯起始因子4A1(EIF4A1)重組蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues:Met1~Thr271
Tags:N-terminal His Tag
Subcellular Location: Cytoplasm
Purity: > 95%
Traits:Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose.
Original Concentration:200µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:4.9
Predicted Molecular Mass:34.7kDa
Accurate Molecular Mass:35kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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