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科學(xué)家發(fā)現(xiàn)了受損DNA乘坐的救護(hù)車
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γ-氨基丁酸A受體α2(gABRa2)重組蛋白 GABA是哺乳動物大腦中主要的抑制性神經(jīng)遞質(zhì),它作用于GABA-A受體,即配體門控的氯離子通道。這些通道的氯電導(dǎo)可以通過與GABA-A受體結(jié)合的苯二氮卓類藥物來調(diào)節(jié)。已經(jīng)鑒定出至少16種不同的GABA-A受體亞基?;蛘?,已經(jīng)發(fā)現(xiàn)該基因存在編碼相同蛋白質(zhì)的剪接轉(zhuǎn)錄變體?!居蒖efSeq提供,2008年7月】
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品牌 | 信裕生物 | CAS | 詳見說明書 |
---|---|---|---|
分子式 | 詳見說明書 | 純度 | > 90% |
分子量 | 54.1kDa | 貨號 | XY92071RPHu01 |
規(guī)格 | 50µg/100µg | 供貨周期 | 一周 |
主要用途 | SDS-PAGE; WB | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
Recombinant Gamma-Aminobutyric Acid A Receptor Alpha 2 (gABRa2)
γ-氨基丁酸A受體α2(gABRa2)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Phe42~Ile250
Tags: N-terminal His and GST Tag
Subcellular Location: Membrane
Purity: > 90%
Traits: Freeze-dried powder
Buffer formulation: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%Trehalose.
Original Concentration: 100µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 7.2
Predicted Molecular Mass: 54.1kDa
Accurate Molecular Mass: 64kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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