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γ-氨基丁酸A受體α2(gABRa2)重組蛋白

γ-氨基丁酸A受體α2(gABRa2)重組蛋白 GABA是哺乳動物大腦中主要的抑制性神經(jīng)遞質(zhì),它作用于GABA-A受體,即配體門控的氯離子通道。這些通道的氯電導(dǎo)可以通過與GABA-A受體結(jié)合的苯二氮卓類藥物來調(diào)節(jié)。已經(jīng)鑒定出至少16種不同的GABA-A受體亞基?;蛘?,已經(jīng)發(fā)現(xiàn)該基因存在編碼相同蛋白質(zhì)的剪接轉(zhuǎn)錄變體?!居蒖efSeq提供,2008年7月】

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詳細(xì)介紹
品牌信裕生物CAS詳見說明書
分子式詳見說明書純度> 90%
分子量54.1kDa貨號XY92071RPHu01
規(guī)格50µg/100µg供貨周期一周
主要用途SDS-PAGE; WB應(yīng)用領(lǐng)域生物產(chǎn)業(yè)

Recombinant Gamma-Aminobutyric Acid A Receptor Alpha 2 (gABRa2) 

γ-氨基丁酸A受體α2(gABRa2)重組蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Phe42~Ile250

Tags: N-terminal His and GST Tag

Subcellular Location: Membrane

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%Trehalose.

Original Concentration: 100µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.2

Predicted Molecular Mass: 54.1kDa

Accurate Molecular Mass: 64kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

            Store at 2-8oC for one month.

            Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

γ-氨基丁酸A受體α2(gABRa2)重組蛋白

[  IDENTIFICATION  ]

γ-氨基丁酸A受體α2(gABRa2)重組蛋白

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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