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實(shí)戰(zhàn)分享|小鼠淋巴細(xì)胞的提取和分選之經(jīng)驗(yàn)小結(jié)
實(shí)驗(yàn)常用人肝癌細(xì)胞株(HepG2/Hep3B,HuH-7,MHCC97H,PLC/PRF/5)怎么選?
原代細(xì)胞的培養(yǎng)方法及注意事項(xiàng)
小鼠D二聚體(D2D)Elisa Kit產(chǎn)品類型:
ELISA Kit
產(chǎn)品名稱:
小鼠D二聚體(D2D)Elisa Kit
英文名稱:
Mouse D-Dimer,D2D ELISA Kit
貨號:
XY-E13584m
規(guī)格:
96T
種屬:
Mouse
待測物名稱:
D-Dimer,D2D
縮寫:
D2D
檢測范圍:
31.25 pg/ml-2000 pg/ml
靈敏度:
7.81 pg/ml
反應(yīng)時間:
1-5h
所需樣本體積:
50-100ul
檢測波長:
450 nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for D2D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any D2D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for D2D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of D2D bound in the initial step. The color development is stopped and the intensity of the color is measured.
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